DNA Sequencing Question (Sanger Method)? - gel band, color meanings
In the sequencing reaction adideoxyDNA, Jessica M. made a mistake and adds 100% ddTTPand 0% dTTPinstead standard rate of 5% ddTTPand dTTP 95%. Compared to a normal reaction of the sequencing, we expect that the products of the sequencing of Jessica when they run on a gel-sequencing to include a ladder sequence (streaks in the sequencing gel), the following:
(A) Normal.
(B) is not normal. Sequencing of the entire T-bands are missing
(C) abnormal. The sequence of steps in which the A, G, C, and the bands are gone
(D) abnormal. Scale very short sequence of a few bands on the bottom of the gel
(E) abnormal. Very long sequence of steps, with additional bands above the freezing point
Monday, January 25, 2010
Gel Band, Color Meanings DNA Sequencing Question (Sanger Method)?
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